Opilio LLC

Tune-A-Melt

Interactive thermal melt curve analysis for raw fluorescence, normalized traces, derivative curves, Tm, and ΔTm.

1. Load melt data

Derivative curves are generated from the raw fluorescence file.

2. Experiment labels

3. Analysis ranges

4. Export results

Exports use current range and selected curves.

No file loaded yet.

Tm values

No Tm values to show.

Curve selection

Click to show/hide. Alt-click, Option-click, or Shift-click a curve to mark it as a control. Multiple controls are averaged for ΔTm.

Upload raw data to populate curve selection.

Comments / processing summary